Elucidation of the mechanism of byproduct DNA synthesis in whole-transcript amplification by poly-A tagging and development of its specific suppression
Yohei Sasagawa, Yoshimi Iwayama, Minoru Yano, Itoshi Nikaido
This paper elucidates the mechanism of byproduct DNA synthesis in poly-A tagging and develops SupTag to suppress it, improving transcriptomic analysis accuracy.
bioRxiv · Aug 19, 2025 · PreprintMolecular Biology