Yohei Sasagawa

Cycling persister clones with elevated NR2F1-mediated cholesterol biosynthesis cause chemotherapy resistance

The paper identifies cycling persisters (CPs) in cancer organoids and shows that elevated NR2F1-mediated cholesterol biosynthesis contributes to chemotherapy resistance.

MitoDelta: identifying mitochondrial DNA deletions at cell-type resolution from single-cell RNA sequencing data

MitoDelta detects mitochondrial DNA deletions at cell-type resolution using single-cell RNA sequencing data, revealing distinct deletion burdens across neuronal subtypes in Parkinson's disease.

Elucidation of the mechanism of byproduct DNA synthesis in whole-transcript amplification by poly-A tagging and development of its specific suppression

This paper elucidates the mechanism of byproduct DNA synthesis in poly-A tagging and develops SupTag to suppress it, improving transcriptomic analysis accuracy.

Distinctiveness and continuity in transcriptome and connectivity in the anterior-posterior axis of the paraventricular nucleus of thalamus

This study identifies four cell types in the mouse PVT using single-cell RNA sequencing and shows their transcriptomic continuity along the anterior-posterior axis, with distinct axon projection patterns and functional responses to neuropeptides.

Local states of chromatin compaction at transcription start sites control transcription levels

The paper identifies local chromatin compaction states at transcription start sites (TSSs) as determinants of transcription levels by correlating weak compaction with RNA polymerase binding.

Benchmarking Single-Cell RNA Sequencing Protocols for Cell Atlas Projects

This study benchmarks 13 scRNA-seq protocols to evaluate their effectiveness in characterizing cell types and states for cell atlas projects.

Quartz-Seq2: a high-throughput single-cell RNA-sequencing method that effectively uses limited sequence reads

Quartz-Seq2 improves single-cell RNA-seq by increasing UMI conversion efficiency and gene detection from limited reads using in vitro and in vivo samples.