Multiplex labeling and manipulation of endogenous neuronal proteins using sequential CRISPR/Cas9 gene editing
Wouter J. Droogers, Jelmer Willems, Harold D. MacGillavry, Arthur P.H. de Jong
This paper introduces CAKE, a method for multiplex CRISPR/Cas9 gene editing in neurons to label and manipulate endogenous proteins with high accuracy and low crosstalk.
eNeuro · Jul 1, 2022Peer-reviewedNeuroscience