Harold D. MacGillavry

Axonal ER tubules regulate local translation via P180/RRBP1-mediated ribosome interactions

This study identifies axonal ER tubules and P180/RRBP1 as regulators of local mRNA translation in axons, showing their role in ribosome interactions and proteome dynamics.

Multiplex labeling and manipulation of endogenous neuronal proteins using sequential CRISPR/Cas9 gene editing

This paper introduces CAKE, a method for multiplex CRISPR/Cas9 gene editing in neurons to label and manipulate endogenous proteins with high accuracy and low crosstalk.

Distinct mechanisms underlie the subsynaptic mobility of presynaptic metabotropic glutamate receptor types to tune receptor activation

This study investigates the subsynaptic mobility of presynaptic mGluR2 and mGluR7, revealing distinct mechanisms that regulate their distribution and impact on synaptic transmission.

Quantitative mapping of transcriptome and proteome dynamics during polarization of human iPSC-derived neurons

The paper quantitatively maps transcriptome and proteome changes during human iPSC-derived neuron polarization, identifying extensive remodeling of gene and protein expression.

ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons

ORANGE enables targeted genomic integration of fluorescent tags in neurons to study endogenous protein distribution with high-resolution imaging.

Temporal quantitative proteomics of protein translation and phosphorylation in synaptic plasticity

The paper uses proteomics and phosphoproteomics to study mGluR-LTD in neurons, identifying key kinases and pathways involved in synaptic plasticity.