A benchmark comparison of CRISPRn guide-RNA design algorithms and generation of small single and dual-targeting libraries to boost screening efficiency
Sebastian Łukasiak, Alex T. Kalinka, Nikhil Gupta, Angelos Papadopoulos, Khalid Saeed, Ultan McDermott, Gregory J. Hannon, Douglas Ross‐Thriepland, David Walter
The paper benchmarks CRISPR sgRNA design algorithms and creates smaller, efficient libraries for loss-of-function screens.
BMC Genomics · Feb 26, 2025Peer-reviewedMolecular Biology