Rutger A. F. Gjaltema

The paper identifies H3K9me3 accumulation at the Xist promoter as a transcription-dependent mechanism that influences random XCI choice in female cells.

The paper shows that antisense transcription can create expression memory through promoter repression in mouse embryonic stem cells, with memory capacity increasing during differentiation.

CasTuner enables analog tuning of endogenous gene expression using CRISPR/Cas systems and a degron domain, showing dose-dependent repression and application in studying gene dosage effects.

The paper identifies distal and proximal cis-regulatory elements that respond to X-chromosomal dosage and developmental state to regulate Xist expression during XCI in mouse embryonic stem cells.