Melissa Bothe

Transcription-dependent heterochromatin at the Xist promoter shapes the random choice of the inactive X chromosome

The paper identifies H3K9me3 accumulation at the Xist promoter as a transcription-dependent mechanism that influences random XCI choice in female cells.

Antisense transcription can induce expression memory via stable promoter repression

The paper shows that antisense transcription can create expression memory through promoter repression in mouse embryonic stem cells, with memory capacity increasing during differentiation.

Reporter CRISPR screens decipher cis- and trans-regulatory principles at the Xist locus

The paper uses CRISPR screens to identify transcription factors regulating Xist expression and their interactions with cis-regulatory elements during X-chromosome inactivation.

Assessment of glucocorticoid-induced enhancer activity of eSNP regions using STARR-seq reveals novel molecular mechanisms in psychiatric disorders

Glucocorticoid receptor activation induces gene-specific transcriptional memory and universally reversible changes in chromatin accessibility

The paper shows that glucocorticoid receptor activation leads to reversible chromatin changes and gene-specific transcriptional memory in human airway epithelial cells.

Androgen and glucocorticoid receptor direct distinct transcriptional programs by receptor-specific and shared DNA binding sites

This study identifies mechanisms by which androgen and glucocorticoid receptors achieve functional diversification through distinct DNA binding and downstream regulatory events.

Synthetic STARR-seq reveals how DNA shape and sequence modulate transcriptional output and noise.

This study develops synSTARR-seq to analyze how DNA sequence and shape affect transcriptional activity and noise using synthetic GR binding sites.