Author

Joseph L. Harman

University of WashingtonORCID 0000-0002-8283-0301Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Synthetic Biology· 2 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 1 Research Focus:database / resource· 1 Assay:protein structure· 1 Organism:yeast· 1

Frequent Co-Authors

Aditya A. Agarwal· 1 shared Adrian W. Lange· 1 shared Alexander EngiD· 1 shared Alfredo Quijano‐RubioiD· 1 shared Brianna Fernandez· 1 shared Charles Lin· 1 shared Colleen Shikany· 1 shared Daniel Cunningham‐BryantiD· 1 shared

Activity by Year

2021-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 17 mutual co-author links

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1 shared paperSynthetic Biology

Aditya A. Agarwal

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3 papers

Recent·Most cited

Synthetic BiologybioRxivPreprintApr 19, 2026

The Synthetic Epitope Atlas: High-Throughput Design and Validation of De Novo Antibody-Antigen Complexes

Nicholas Altieri, Joseph L. Harman, David Noble…·Adrian W. Lange

The paper generates and validates synthetic epitope proteins to improve de novo antibody design by combining computational design with high-throughput experimental binding data.

1 citation

protein structureexperimental assay

Molecular BiologybioRxivPreprintJul 21, 2025

LuxSit Pro and sPro: Next-Generation Designed Luciferases for Bioluminescent Reporting and Complementation-Based Detection

Renan Vergara, Jonathan Wagner, Paursa Kamalian…·Daniel‐Adriano Silva

The paper introduces LuxSit Pro and sPro, next-generation luciferases with enhanced brightness, stability, and versatility for bioluminescent reporting and molecular interaction detection.

5 citations

experimental assay

Synthetic BiologyACS Synthetic BiologyPeer-reviewedNov 1, 2021

CRISPR-Cas-mediated tethering recruits the yeast HMR mating-type locus to the nuclear periphery but fails to silence gene expression

Emily R. Cliff, Robin L. Kirkpatrick, Daniel Cunningham‐Bryant…·Jesse G. Zalatan

This study shows that CRISPR-Cas-mediated tethering recruits the yeast HMR locus to the nuclear periphery but does not silence gene expression, unlike a Gal4-mediated system.

DOI 10.1021/acssynbio.1c00306

yeastexperimental assay