Author

Isabel Pardo

Novo Nordisk FoundationORCID 0000-0002-8568-1559Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:protein design· 2 Approach:experimental assay· 1 Approach:statistical model· 1

Frequent Co-Authors

Daniel C. VolkeiD· 2 shared Jiřı́ DamborskýiD· 2 shared Pablo I. NikeliD· 2 shared Alexander Kai BuelliD· 1 shared Antonín KunkaiD· 1 shared Anuj ShettyiD· 1 shared David BednářiD· 1 shared Gladstone G. SigamaniiD· 1 shared

Activity by Year

2022-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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2 shared papersBiochemistry

Daniel C. VolkeORCID

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2 papers

Recent·Most cited

BiochemistrybioRxivPreprintJul 28, 2025

Mechanism-Guided Engineering of Fluorinase Unlocks EfficientNucleophilic Biofluorination

Michaela Slanska, Daniel C. Volke, Isabel Pardo…·Zbyněk Prokop

This paper enhances fluorinase catalytic efficiency by engineering mutants and optimizing reaction conditions, achieving a 60-fold increase in turnover rate.

1 citation

statistical modelprotein design

Synthetic BiologyACS CatalysisPeer-reviewedMay 19, 2022

A non-conventional archaeal fluorinase identified by in silico mining catalyzes the fastest natural nucleophilic fluorination

Isabel Pardo, David Bednář, Patricia Calero…·Pablo I. Nikel

The paper identifies a novel archaeal fluorinase with the highest reported fluorination rate and demonstrates its application in enhancing fluoronucleotide production in bacterial hosts.

61 citationstop 4%

experimental assayprotein design