Mechanism-Guided Engineering of Fluorinase Unlocks EfficientNucleophilic Biofluorination
Michaela Slanska, Daniel C. Volke, Isabel Pardo, Antonín Kunka, Naveen Banchallihundi Krishna, Anuj Shetty, Likith Muthuraj, Gladstone G. Sigamani, Roopa Lalitha, Alexander Kai Buell, Martin Marek, R. Pravin Kumar, Jiřı́ Damborský, Pablo I. Nikel, Zbyněk Prokop
This paper enhances fluorinase catalytic efficiency by engineering mutants and optimizing reaction conditions, achieving a 60-fold increase in turnover rate.
bioRxiv · Jul 28, 2025 · PreprintBiochemistry