Keiji Nishida

LsBADH1 is responsible for sweet fragrance in lettuce (Lactuca sativa L.) through 2-acetyl-1-pyrroline biosynthesis

The paper identifies LsBADH1 as the gene responsible for 2-acetyl-1-pyrroline (2AP) biosynthesis, which causes the sweet fragrance in lettuce, and shows that a premature stop codon in LsBADH1 increases 2AP levels.

Development of a highly efficient base editing system for Lactobacilli to improve probiotics and dissect essential functions

The paper develops a base editing system for Lactobacilli to introduce precise point mutations without donor DNA, enabling functional studies and strain improvement.

LsTT2 encoding R2R3-MYB transcription factor is responsible for a shift from black to white in lettuce seed

This study identifies LsTT2, an R2R3-MYB transcription factor gene, as responsible for the shift from black to white seed color in lettuce through genetic mapping and genome editing validation.

A multi-kingdom genetic barcoding system for precise target clone isolation

This paper introduces CloneSelect, a multi-kingdom genetic barcoding system that enables precise isolation of target clones using CRISPR base editing to trigger reporter gene expression.