Computationally inspired glycoengineering to maximise mAb β4-galactosylation
Itzcóatl A. Gómez, Mina Ghahremanzamaneh, Apostolos Tsopanoglou…·Ioscani Jiménez del Val
This paper describes a dual genetic engineering strategy to enhance mAb β4-galactosylation in CHO cells, achieving over 90% galactosylation and reducing Man5 glycans without affecting mAb titre.