Julian MJ Pietsch

A toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of the second messenger regulatory network

The paper develops FRET-based c-di-GMP biosensors and applies them with FRET-To-Sort to map regulatory networks controlling c-di-GMP levels in E. coli.

A label-free method to track individuals and lineages of budding cells

This paper introduces BABY, a label-free method to track budding yeast cells and estimate growth rates using machine learning, revealing sizer- and timer-controlled bud growth and Sfp1 dynamics.

Distributed and dynamic intracellular organization of extracellular information

The paper quantifies how extracellular information is encoded in the dynamic nuclear localization of transcription factors in yeast, showing that collective dynamics, not individual factors, represent environmental states.