Randall H. Morse

The paper examines Mediator complex dynamics during heat shock in yeast, finding altered occupancy and interactions with PIC components at stress-responsive genes.

The paper shows that Kin28 depletion increases Taf1 and Taf4 promoter occupancy in yeast, while TBP occupancy remains unchanged, suggesting uncoupling of Taf and TBP during transcription.

The paper investigates Mediator complex recruitment and dynamics in yeast, showing that Mediator lacking Med2-Med3-Med15 is associated with active promoters and that PIC components like TBP, Pol II, and Taf1 influence Mediator association.

This study shows that Mediator subunits regulate Ty1 retrotransposon mobility by altering the ratio of Ty1i to Ty1 transcripts, affecting retromobility without significantly changing genomic RNA or Gag levels.