Jonathan Alles

Open-ST provides a high-resolution, 3D spatial transcriptomics method to study tissue molecular organization, revealing spatially contiguous structures and biomarkers in tumors and lymph nodes.

The paper quantifies nuclear and cytoplasmic poly(A) tail dynamics, showing rapid nuclear deadenylation and distinct tail lengths for different transcripts.

The paper introduces FLAM-seq, a method for full-length mRNA sequencing including poly(A) tails, and finds correlations between 3' UTR length, polyadenylation sites, and tail composition.

The paper generates a single-cell transcriptomic map of Drosophila embryos, identifies spatial gene expression patterns, and discovers patterned long non-coding RNAs.

This paper introduces a method for fixing and preserving cells for single-cell transcriptomics, demonstrating its effectiveness across species and tissue types.