Ivano Legnini

The human RNA-DNA interactome is cell type-specific and dynamic

The paper maps RNA-DNA interactions in 16 human cell types, showing cell-type-specific and dynamic networks that correlate with gene regulation and disease traits.

Insulin synthesis is sustained by Tent5 poly(A) polymerases

The paper identifies Tent5 poly(A) polymerases as stabilizers of insulin mRNA, showing their specific activity regulated by glucose and localization.

MicroRNAs are required within a critical time window to define neural patterning during early human brain development

The paper shows miRNAs are essential during a specific time window for proper neural patterning in human brain development, with disruption causing defects in forebrain identity.

Genome Architecture Shapes Transcriptional Responses to DNASupercoiling in a Multicellular Organism

This study shows DNA supercoiling affects transcription initiation and elongation in C. elegans, with directional impacts on gene expression based on genomic context.

Genomic codes governing enhancer RNA fate

This paper introduces CFC-seq and SALA to sequence non-poly(A) RNAs, identifies 39,425 novel transcriptional units, and reveals a genomic code governing enhancer RNA fate based on promoter architecture.

Mutant Huntingtin impairs neurodevelopment in human brain organoids through CHCHD2-mediated neurometabolic failure

This study shows that mutant Huntingtin causes neurodevelopmental issues in human brain organoids by disrupting CHCHD2, leading to mitochondrial and metabolic problems.

High-resolution molecular atlas of a lung tumor in 3D

This paper presents a 3D spatial atlas of a human lung carcinoma, revealing tumor microenvironment niches and their associated immune interactions and survival outcomes.

Rapid nuclear deadenylation of mammalian messenger RNA

The paper quantifies nuclear and cytoplasmic poly(A) tail dynamics, showing rapid nuclear deadenylation and distinct tail lengths for different transcripts.

Neurodegeneration in human brain organoids infected with herpes simplex virus type 1

This study infects human brain organoids with HSV-1 and identifies molecular and cellular changes associated with neurodegeneration.

Bulk and single-cell gene expression profiling of SARS-CoV-2 infected human cell lines identifies molecular targets for therapeutic intervention

The paper uses bulk and single-cell transcriptomics to analyze SARS-CoV-2 infection in human cell lines, identifying HSP90 as a potential therapeutic target.

Full-length mRNA sequencing reveals principles of poly(A) tail length control

The paper introduces FLAM-seq, a method for full-length mRNA sequencing including poly(A) tails, and finds correlations between 3' UTR length, polyadenylation sites, and tail composition.

Dysregulation of circular RNAs in myotonic dystrophy type 1

This study identifies dysregulated circular RNAs in myotonic dystrophy type 1 patients using RNA-sequencing and qPCR, showing tissue-specific expression patterns and potential as biomarkers.