Hamidreza Ebrahimiyan

The paper compares SYBR Green and Probe-based Q-PCR methods for measuring vector copy numbers and viral titration in CAR T cell products, finding Probe has lower contamination dependency.

This study evaluates marker rescue and qPCR assays for detecting replication-competent retrovirus in gene therapy products, showing improved sensitivity with amplification steps.

The paper develops an ER retrieval-and-release system to study ligand-independent basal TCR signaling, finding that surface TCRs generate distinct phosphorylation patterns compared to ligand-induced activation.

This paper develops a method to reduce TCR expression in CAR T cells using engineered CD3 subunits with an endoplasmic reticulum retention signal, avoiding genome editing and enabling safer allogeneic CAR T cell production.