Halina Mikolajek

This paper uses photocaged molecular oxygen to trigger oxygen binding in myoglobin crystals and captures structural changes using time-resolved serial X-ray crystallography.

The paper describes the engineering of mCoral, a modified mCherry variant with improved hydrogen peroxide tolerance and pH responsiveness, and elucidates its structural and dynamic properties.

This study shows that shipping protein crystals at room temperature across long distances does not compromise diffraction quality for in situ X-ray diffraction experiments.