Cynthia I. Terrace

This study investigates the trade-off between activity and specificity in adenine base editors using machine learning and high-throughput screening, finding that activity is the primary determinant of editing outcomes.

Tobacco rattle virus delivery of engineered ISDra2 variant eTnpBc and its guide RNA as a single transcript achieves up to 90% somatic and 100% heritable editing in Nicotiana benthamiana.

The paper introduces SABER, a method to efficiently map tolerated deletions in SaCas9, enabling the design of minimal CRISPRi effectors.

This study identifies activating mutations in TnpB enzymes that enhance genome editing activity across multiple species.