The paper identifies T-dioxygenases in bacteriophages, revealing a subclass requiring a regulator protein for oxidation and structural insights into their function.

DOI 10.1101/2025.08.14.668559

bacteria (other)proteomics

𝕏 PostBluesky

BiochemistryPLoS ONEPeer-reviewedDec 2, 2024

High-throughput Kinetics using Capillary Electrophoresis and Robotics (HiKER) platform used to Study T7, T3, and Sp6 RNA Polymerase Misincorporation

Zachariah I. Carter, William B. O’Brien, Sean R. Lund, Andrew F. Gardner

The paper introduces HiKER, a high-throughput platform for measuring RNA polymerase kinetics, showing differences in misincorporation rates among T7, T3, and Sp6 RNAPs.

4 citations

proteomicsexperimental assay

𝕏 PostBluesky

Synthetic BiologyACS Synthetic BiologyPeer-reviewedFeb 20, 2024

Highly parallelized construction of DNA from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate

Sean R. Lund, Vladimir Potapov, Sean R. Johnson…·Nathan A. Tanner

The paper describes a method for efficiently constructing genes from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate Assembly.

18 citations

experimental assay

𝕏 PostBluesky