Azeet Narayan

MEDUSA improves sequencing accuracy by minimizing DNA duplex resynthesis during library preparation, achieving lower residual SNV frequency in genomic and cell-free DNA.

This study benchmarks six duplex sequencing technologies to evaluate their performance in detecting somatic mutations across different genomic contexts and tissue types.

The paper benchmarks somatic variant and indel detection at low allele fractions using short- and long-read data, finding sensitivity and precision around 80% at 300x coverage.

Methyl-CODEC enables simultaneous methylation and duplex sequencing, improving mutation detection and distinguishing methylated Cs from mutations.