Tom F. A. de Greef

DIALECT: DNA-Programmable Synthetic Cells Communicate with Mammalian Cells through Synthetic Receptors

DIALECT enables synthetic cells to communicate with mammalian cells via DNA-programmed ligand release and engineered receptors, achieving specific signaling and logic gate functions.

Molecular access control for random-access DNA data storage

This paper introduces DNA-GUARD, a molecular access-control system for DNA data storage that restricts PCR-based retrieval and complements encryption.

Engineered microRNA feedback circuits enable tunable and autonomous control of synthetic receptor activity

This paper introduces a miRNA feedback circuit to control synthetic receptor activity, showing tunable and autonomous regulation of synNotch receptor expression.

Unveiling DNA Origami Interaction Dynamics on Living Cell Surfaces by Single Particle Tracking

This study uses single particle tracking to analyze DNA origami interaction dynamics with cancer cells, revealing ligand-specific binding and density-dependent behavior.

Engineering a Scalable and Orthogonal Platform for Synthetic Communication in Mammalian Cells

This paper develops a scalable synthetic communication platform for mammalian cells using orthogonal receptors and coiled-coil peptides to enable logic gate computations in cell consortia.

DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access

This paper develops thermoresponsive microcapsules for repeated random access of DNA data, demonstrating improved performance over non-compartmentalized storage.

Determinants of ligand-functionalized DNA nanostructure-cell interactions

This study investigates how design parameters affect the binding of antibody-functionalized DNA nanostructures to cell surface receptors, finding that nanostructure size and DNA handle location influence receptor binding.

Cell-Free Characterization of Coherent Feed-Forward Loop-Based Synthetic Genetic Circuit

The paper characterizes a synthetic coherent feed-forward loop (CFFL) in E. coli cell-free systems, showing it can repress background activity but is not effective as a temporal filter.

Light-activated signaling in DNA-encoded sender-receiver architectures

The paper develops DNA-encoded sender-receiver architectures using microfluidic trapping arrays and light-activated DNA strand displacement to study diffusion-based signaling and spatial integration of chemical communication.

Engineered Living Materials based on Adhesin-mediated Trapping of Programmable Cells

This paper introduces a living material platform that traps engineered E. coli in hydrogels using adhesin-mediated anchoring, reducing bacterial leakage and enabling controlled release of antimicrobial proteins.

A DNA-based synthetic apoptosome

This paper describes a DNA-based synthetic apoptosome that demonstrates proximity-driven enzymatic activity and provides insights into caspase-9 regulation.

Minimally Complex Nucleic Acid Feedback Control Systems for First Experimental Implementations

This paper presents minimally complex DNA-based control circuits for experimental implementation of feedback control systems in synthetic biology.

Distributed DNA-based Communication in Populations of Synthetic Protocells

This paper introduces a platform for distributed DNA-based communication in synthetic protocells using DNA strand-displacement circuits.