Matteo Ciciani

The paper describes the development of En-ISPmu1, a modified TnpB nuclease, and demonstrates its ability to achieve targeted genome integration in mouse liver with a single AAV vector.

The paper enhances Cas{Phi}2 activity using EPICA.2, an optimized directed evolution platform, resulting in evoCas{Phi}2 with 70-fold increased activity in human cells.

This study uses CRISPR base editing to introduce revertant mutations in F508del-CFTR, rescuing protein folding and function in CF patient-derived cells.

The paper presents a method to discover new human gut viruses using enriched virome samples and reports over 162,000 novel viral sequences.