Astrid Wirtz

Microbial production of the low-caloric sweetener D-allulose from D-glucose by evolutionary engineering

This paper evolves microbial strains to efficiently produce D-allulose from D-glucose, achieving a 15% yield at 30°C using engineered enzymes and a chassis strain.

Metabolic engineering of Corynebacterium glutamicum for production of the low-caloric natural sweetener D-allulose via phosphorylated intermediates

This paper engineers Corynebacterium glutamicum to produce D-allulose via a phosphorylation-dephosphorylation pathway, achieving a 2.3-fold increase in production in minimal medium.