Author

Nicholas J. Kapolka

ORCID 0000-0003-2223-8207Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 1 Approach:experimental assay· 1 Organism:human· 1 Research Focus:protein design· 1 Organism:yeast· 1

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Bruno ColoniD· 1 shared Daniel G. IsomiD· 1 shared Jacinda PujolsiD· 1 shared Kyutae David LeeiD· 1 shared Sam TayloriD· 1 shared

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2026-2026 · peak 1/yr

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5 top collaborators · 10 mutual co-author links

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1 shared paperSynthetic Biology

Bruno ColonORCID

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1 paper

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Synthetic BiologyJournal of Biological ChemistryPeer-reviewedOct 1, 2026

Systematic mapping of chimera-permissive sites by CRISPR-guided PAM scanning

Jacinda Pujols, Kyutae David Lee, Nicholas J. Kapolka…·Daniel G. Isom

This paper uses CRISPR-guided PAM scanning to identify chimera-permissive sites in proteins, enabling the design of functional chimeras with preserved activity.

DOI 10.1016/j.jbc.2026.113627

yeasthuman

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