Daniel G. Isom

This paper uses CRISPR-guided PAM scanning to identify chimera-permissive sites in proteins, enabling the design of functional chimeras with preserved activity.

This study identifies structural features of the A2AR receptor that enable pH sensing without the typical acidic triad, showing mutations can eliminate pH sensitivity while preserving agonist signaling.

This study shows opioid receptors act as proton-gated coincidence detectors, with a conserved aspartic acid residue critical for pH sensing and agonist activation.

This paper develops ORION, a genetically encoded BRET pH sensor with improved performance and broader pH range, and demonstrates its use in identifying acid imprinting states in cancer cells.

This paper describes a rapid CRISPR-Cas9 genome editing protocol for S. cerevisiae using PCR-based guide installation and seamless plasmid recircularization.

The paper examines non-redundant roles of paralogous proteins in yeast glucose-sensing pathways through gene deletion and multi-omics analyses.

PIONEER is a yeast-based platform for screening genetically encoded protein regulators by displaying them in the periplasmic space to assess their function through signaling.

The paper analyzes 223,000 SARS-CoV-2 proteome sequences to track mutations over one year, identifying distinct mutation patterns in different time periods and geographical regions.