Kyutae David Lee

Systematic mapping of chimera-permissive sites by CRISPR-guided PAM scanning

This paper uses CRISPR-guided PAM scanning to identify chimera-permissive sites in proteins, enabling the design of functional chimeras with preserved activity.

Modulating proton sensing by the adenosine receptor A2

This study identifies structural features of the A2AR receptor that enable pH sensing without the typical acidic triad, showing mutations can eliminate pH sensitivity while preserving agonist signaling.

Proton-gating of opioid receptors and isoform functionality

This study shows opioid receptors act as proton-gated coincidence detectors, with a conserved aspartic acid residue critical for pH sensing and agonist activation.

Promoter- and Enhancer-Dependent Cohesin Loading Initiates Chromosome Looping to Fold Tcrb Loci for Long-Range Recombination

The paper shows that cohesin loading from Vβ promoters or enhancers initiates loop extrusion across the Tcrb locus, enabling long-range recombination.