Sergei V. German

This paper demonstrates the successful restoration of valine biosynthesis in mammalian cells by integrating synthetic pathways from E. coli, showing that ancient metabolic pathways can be reactivated in metazoans.

MenDEL is a web tool that automatically designs PCR primers for DNA assemblies by optimizing primer pairs for each junction based on user constraints.

MenDEL automates the selection of BAC sets to cover long DNA regions by allowing users to define criteria and find optimal combinations from existing libraries.

The paper uses synthetic genomic reconstitution to study Hox cluster regulation, finding that distal enhancers are required for full transcriptional output while chromatin remodeling can occur without them.

The paper introduces a method to assemble synthetic haplotypes for functional analysis of AMD-associated variants, demonstrating its application to the 10q26 risk region.