Hsin-Ho Huang

This paper designs an incoherent merger network in E. coli to achieve a ratiometric gene expression response that is linear, tunable, and robust to cellular perturbations.

This paper introduces a feedforward controller that compensates for growth rate defects caused by heterologous gene activation in bacteria.

This paper introduces a dCas9 regulator that reduces competition among sgRNAs in CRISPRi circuits, enabling independent gene repression control.

This paper designs a quasi-integral feedback controller to maintain gene expression levels despite changes in ribosome availability, showing it prevents expression reduction when a resource competitor is activated.

The paper investigates how resource competition affects genetic circuits in E. coli and provides a model to mitigate unintended behaviors by tuning resource demand coefficients.