Patrick J. Byrne

This study evaluates alternative dyes for image-based profiling assays, finding that some substitutions maintain performance while enabling live-cell imaging and expanded feature space.

The paper presents a comprehensive genetic perturbation dataset using Cell Painting to analyze gene expression effects in human cells, revealing novel gene clusters and functional relationships.

The paper updates the Cell Painting assay protocol to improve morphological profiling, finding that simplified steps and reduced stain concentrations maintain robust outputs.

Cell Painting images and profiles for 115,796 compounds in U2OS cells from 12 laboratories; 15.2% of analyzed compounds are phenotypically active, and cross-laboratory replicates cut reference-compound activity by 34%.

This study identifies Sp7 and its target gene osteocrin as critical for osteocyte dendrite formation and shows their role in human skeletal diseases.