Pascal Sirand‐Pugnet

BoviScan is a PhIP-Seq library that maps antibody responses against Mycoplasma bovis, enabling high-throughput and high-resolution analysis of humoral immunity.

The paper improves a CRISPR-base editor tool for targeting virulence factors in Mycoplasma bovis by enabling multi-loci targeting and expanding targetable loci.

The paper shows that an atypical F-type ATPase is required for the MIB-MIP system's function in mycoplasmas to evade immune agglutination.

The paper uses gene content analysis to classify bacterial genera within the Mycoplasmatales-Entomoplasmatales clade, identifying issues with recent taxonomic revisions.

The paper introduces a new method combining Fusion cloning and CRISPR-Cas9 to clone and edit large bacterial genomes in yeast, demonstrating its effectiveness with Mycoplasma species.