Takeharu Nagai

The paper develops an ATP-free bioluminescence system using DOX to convert fatty acids to fatty aldehydes, enhancing stability under metabolic stress.

This paper introduces 2P-RESOLFT microscopy, achieving 205-nm lateral resolution to visualize previously unreported membrane protrusions in pancreatic beta cells.

This paper enhances Cas12a's trans-cleavage activity by fusing Sso7d, improving sensitivity and kinetics for nucleic-acid detection.

The paper identifies feature selectivity in M1 melanopsin photoreceptors for circadian regulation, showing their role in encoding light features that synchronize with the day-night cycle.

This study shows that influenza A virus uses intercellular calcium waves triggered by ADP and M2 protein to spread infection, identifying a potential target for prevention.

The paper introduces a high-resolution volumetric imaging system for observing cellular dynamics in large 3D tissues and embryos, demonstrating its application in mouse brain and quail embryo studies.

The paper introduces a method to expand color variation in bioluminescence by tuning dual-acceptor BRET, enabling up to 20 colors for simultaneous bioimaging.

The paper develops a multicolor auto-bioluminescence system for use in bacteria, mammalian, and plant hosts, enabling long-term imaging without external substrates.

The paper uses computational methods to analyze neural activity in the mouse dmPFC during fear conditioning, revealing learning-dependent changes in network topology and hub formation.

The paper visualizes homophilic interactions of clustered protocadherin in neurons using a novel FRET probe and finds that these interactions occur at contact sites between neurites and are stable, while synapses show rare homophilic interactions.

The paper investigates the mechanisms of spiral wave self-organization in social amoebae using trans-scale imaging, identifying population-level, cell-level, and intermediate-scale dynamics.

The paper develops a bioluminescent opsin (bMCO-11) to monitor visual cortical activity in mice without external excitation, enabling continuous tracking of neural responses to optical stimulation.

The paper introduces a new method to record brain activity in freely moving, socially interacting mice using a bioluminescent voltage indicator and a fiber-free technique.