John R. Seavitt

The paper analyzes 3,313 genetically engineered mouse lines to identify gene essentiality as the main factor affecting Cas9-based genome editing success.

The paper analyzes whole-genome sequencing data from Cas9-edited mice to assess off-target mutagenesis, finding minimal off-target activity across 163 guide RNAs.

The paper identifies novel Mendelian genes by analyzing mouse embryo lethality and linking them to human disease phenotypes and genetic variants.

The paper reports on the occurrence of tarsal injuries in male C57BL/6N mice across multiple international facilities, describing clinical signs and findings from radiographic and histological analyses.

This study tests CRISPR/Cas9 with ssODNs and lssODNs for generating conditional knockout alleles in mice, finding higher efficiency with lssODNs.