Evan Tjeerdema

Efficient Endogenous Tagging in the Sea Urchin, Lytechinus pictus, Using CRISPR/Cas9-mediated Split-Fluorescent Protein Knock-In

The paper develops a CRISPR/Cas9-based method for efficient fluorescent protein knock-in in sea urchins by using a split-fluorescent protein strategy, achieving higher integration efficiency than conventional methods.

Automated, high-throughput in-situ hybridization of Lytechinus pictus embryos

This study develops an automated high-throughput HCR pipeline to rapidly map expression of 101 genes in sea urchin embryos, enabling large-scale discovery of novel developmental gene expression patterns.

Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin, Lytechinus pictus.

This paper establishes the first germline transgenic sea urchin using the Minos transposon, enabling stable transgene integration and inheritance.