Amro M. Hamdoun

The paper develops a CRISPR/Cas9-based method for efficient fluorescent protein knock-in in sea urchins by using a split-fluorescent protein strategy, achieving higher integration efficiency than conventional methods.

This paper describes a method to cryopreserve late gastrulae embryos of the sea urchin Lytechinus pictus, enabling their survival through metamorphosis into juvenile adults.

This study creates a single-cell atlas of gene expression, transcription, and chromatin accessibility in sea urchin embryos, revealing dynamic regulatory elements and lineage-specific gene activation patterns.

This study develops an automated high-throughput HCR pipeline to rapidly map expression of 101 genes in sea urchin embryos, enabling large-scale discovery of novel developmental gene expression patterns.

This paper establishes the first germline transgenic sea urchin using the Minos transposon, enabling stable transgene integration and inheritance.

The paper investigates how cell density and proliferation affect 3D cell packing in a sea star embryo, showing that tissue compaction and cell divisions drive scutoid formation.

The paper develops methods to cryopreserve sperm from two sea urchin species, maintaining fertilization capacity and producing viable larvae after multiple freeze-thaw cycles.