Ligang Wu

The paper introduces 4NBoost, a sncRNA-seq protocol with spike-ins to improve quantification, and establishes a reference atlas revealing biases in miRNA and piRNA abundance across tissues and cell lines.

The paper profiles small and long RNA in human oocytes across stages, showing short-piRNAs broadly suppress transposons while long-piRNAs target specific ERVs.

This study shows that piRNAs are essential for generating functional oocytes in golden hamsters, with defects leading to sterility and embryonic arrest.