Molecular BiologybioRxivPreprint
Targeted sequencing of mutations via RNA-templated gap filling of oligonucleotides for single-cell RNA-seq
Mirca Saras Saurty-Seerunghen, Hower Lee, Martha Holdar…·Marco Grillo
This paper introduces a method for targeted sequencing of expressed mutations in single cells using RNA-templated gap filling with BstFL polymerase, enabling simultaneous transcriptomic and mutation profiling.
DOI 10.64898/2026.04.10.717677