Kellie Wise

This paper introduces a method for targeted sequencing of expressed mutations in single cells using RNA-templated gap filling with BstFL polymerase, enabling simultaneous transcriptomic and mutation profiling.

STAMP enables low-cost, high-throughput single-cell analysis by integrating transcriptomics and proteomics imaging, supporting both single and multimodal profiling of diverse cell types.

FixNCut is a reversible fixation and dissociation method that preserves RNA integrity and cellular composition in human and mouse tissues, enabling robust single-cell and spatial analyses.

Head-to-head on serial prostate adenocarcinoma sections: Xenium gave higher sensitivity, wider dynamic range and closer agreement with snRNA-seq; CosMx segmented cells better out of the box.

This study introduces a method to generate high-quality single-nucleus transcriptomic data from FFPE samples using recent single-cell genomic technology.

STOmics-GenX improves gene detection in spatial transcriptomics by integrating CRISPRclean with Stereo-seq, enhancing cell type annotation and biomarker identification in hepatocellular carcinoma.