Targeted sequencing of mutations via RNA-templated gap filling of oligonucleotides for single-cell RNA-seq
Mirca Saras Saurty-Seerunghen, Hower Lee, Martha Holdar, Kellie Wise, Michael J. Roach, Sara Moein, Ted Kang, Taobo Hu, Mats F. Nilsson, Luciano Gastón Martelotto, Anna S. Nam, Marco Grillo
This paper introduces a method for targeted sequencing of expressed mutations in single cells using RNA-templated gap filling with BstFL polymerase, enabling simultaneous transcriptomic and mutation profiling.
bioRxiv · Apr 12, 2026 · PreprintMolecular Biology