Author

Lucas Souza de

Universidade Estadual de Campinas (UNICAMP)ORCID 0000-0003-4468-7441Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Genetics· 1 Genomics· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Assay:CRISPR screen· 2 Research Focus:gene regulatory network inference· 2 Research Focus:variant calling· 2 Organism:yeast· 2 Research Focus:genome assembly· 1 Research Focus:perturbation response prediction· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Ana Paula JacobusiD· 3 shared Jeferson GrossiD· 3 shared Joneclei Alves BarretoiD· 2 shared Leandro Vieira dos SantosiD· 2 shared Danieli Fernanda Canaver MariniD· 1 shared Diego Trindade de SouzaiD· 1 shared Dione Oliveira JordaniD· 1 shared Gabriel Rodrigues Alves MargaridoiD· 1 shared

Activity by Year

2022-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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3 shared papersGenetics

Ana Paula JacobusORCID

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3 papers

Recent·Most cited

GeneticsbioRxivPreprintApr 18, 2026

EasyCAPS: A web tool for restriction-based genotyping and rational CRISPR-Cas9 donor design

Lucas Souza de, Jeferson Gross, Ana Paula Jacobus

EasyCAPS is a web tool that integrates dCAPS primer design with CRISPR-Cas9 strategies to improve genotyping and gene editing efficiency.

DOI 10.64898/2026.04.17.719238

CRISPR screenexperimental assay

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GenomicsBiotechnology for Biofuels and BioproductsPeer-reviewedFeb 12, 2026

QTL mapping, breeding, and debugging Saccharomyces cerevisiae strains through Reiterated Mass Selection and backcrosSing (ReMaSSing)

Lucas Souza de, Joneclei Alves Barreto, Diego Trindade de Souza…·Ana Paula Jacobus

This paper introduces ReMaSSing, a method to identify and correct adaptive alleles in Saccharomyces cerevisiae for improved lignocellulosic hydrolysate tolerance through QTL mapping and breeding.

3 citationstop 4%

yeastexperimental assay

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Synthetic BiologyACS Synthetic BiologyPeer-reviewedOct 18, 2022

EasyGuide plasmids support in vivo assembly of gRNAs for CRISPR/Cas9 applications in Saccharomyces cerevisiae

Ana Paula Jacobus, Joneclei Alves Barreto, Lucas Souza de…·Jeferson Gross

The paper introduces EasyGuide plasmids to simplify CRISPR/Cas9 gRNA assembly in yeast by enabling in vivo gRNA generation directly in yeast.

17 citations

yeastCRISPR screen

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