A programmable, selection-free CRISPR interference system in Staphylococcus aureus
Roni Miah, Mona Johannessen, Morten Kjos, Christian Stephan Lentz
This paper develops a selection-free CRISPRi system in S. aureus that silences virulence genes without antibiotic selection or exogenous inducers, validated in vitro and in vivo.