Cassandra Schaening-Burgos

The paper identifies RluA as the main pseudouridine synthase in E. coli mRNAs, showing it modifies 31 of 44 high-confidence sites through a sequence and structural motif.

This study shows that pseudouridine is added co-transcriptionally to pre-mRNA and affects alternative splicing by modifying RNA structure and interactions.

The paper identifies 2′-O-methyl ribose (Nm) as a prevalent mRNA modification in yeast, showing that Spb1 methylates ribosomal protein mRNAs and that its expression is required for normal levels of target mRNAs.