Wendy V. Gilbert

Identification of rare pseudouridylated microRNA by comprehensive small RNA bisulfite sequencing of mouse and human tissues

The paper identifies rare pseudouridylated microRNA using bisulfite sequencing in mouse and human tissues, finding miR-3068-5p as a modified miRNA with altered target repression.

An atypical E3 ligase safeguards the ribosome during nutrient stress

This study identifies HOIL-1 as an atypical E3 ligase that protects the ribosome during nutrient stress, revealing a new signaling pathway involved in cell fate decisions.

Investigation of TRMT61B methyltransferase activity on mRNA and its effects on translation

This study identifies TRMT61B as an m1A methyltransferase active on cytosolic mRNAs and shows context-dependent effects of m1A on translation and ribosome recruitment.

Small nuclear RNAs enhance protein-free RNA-programmable base conversion on mammalian coding transcripts

This study shows that guided A>I snRNAs improve RNA base conversion efficiency and reduce off-target effects compared to ADAR-recruiting circular RNAs in human cells.

Cellular translational enhancer elements that recruit eukaryotic initiation factor 3

The paper identifies a specific 5'-UTR motif, AMAYAA, that eIF3 binds to, enhancing translation under stress conditions.

Quantitative profiling of human translation initiation reveals regulatory elements that potently affect endogenous and therapeutically modified mRNAs

The paper quantifies translation initiation on human mRNAs, identifies regulatory elements with potent effects, and shows that N1-methylpseudouridine enhances translation in specific 5' UTRs.

RluA is the major mRNA pseudouridine synthase in Escherichia coli

The paper identifies RluA as the main pseudouridine synthase in E. coli mRNAs, showing it modifies 31 of 44 high-confidence sites through a sequence and structural motif.

Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiation

The paper introduces DART, a method to analyze ribosome targeting, revealing thousand-fold differences in 5' UTR-specific translation activity and identifying regulatory elements.

Restriction of SARS-CoV-2 Replication by Targeting Programmed -1 Ribosomal Frameshifting In Vitro

This study identifies merafloxacin as a -1 PRF inhibitor that impedes SARS-CoV-2 replication in vitro.

Pseudouridine synthases modify human pre-mRNA co-transcriptionally and affect splicing

This study shows that pseudouridine is added co-transcriptionally to pre-mRNA and affects alternative splicing by modifying RNA structure and interactions.

Structure and function of yeast Lso2 and human CCDC124 bound to hibernating ribosomes.

The paper presents cryo-EM structures of hibernating yeast and human ribosomes, showing Lso2/CCDC124 binds in a non-rotated state, enabling rapid translation reactivation.

Conserved Methyltransferase Spb1 Targets mRNAs for Regulated Modification with 2′-O-Methyl Ribose

The paper identifies 2′-O-methyl ribose (Nm) as a prevalent mRNA modification in yeast, showing that Spb1 methylates ribosomal protein mRNAs and that its expression is required for normal levels of target mRNAs.