Molecular BiologyNature BiotechnologyPeer-reviewed
Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
Stephen K. Jones, John A. Hawkins, Nicole V. Johnson…·Ilya J. Finkelstein
The paper profiles cleavage specificity and kinetics of CRISPR nucleases using NucleaSeq and CHAMP, finding engineered Cas9s show improved specificity while wtCas12a has similar specificity to wtCas9 in vitro.
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