Kuang Hu

The paper demonstrates that half-match recombination, independent of target-site complementarity, drives bridge RNA-guided excision and off-target insertion in IS621 recombination.

The paper redefines IS110 as a dynamic platform capable of expanding into large elements that disseminate antimicrobial resistance.

This paper discovers and engineers retron-based gene editors for precise genome editing in mammalian cells and vertebrates, achieving high efficiency with novel reverse transcriptases and delivery strategies.

This study identifies distinct horizontal transfer mechanisms for type I and type V CRISPR-associated transposons, showing they use CRISPR RNAs and alternative unguided mechanisms for gene transfer.

The paper identifies new CRISPR-associated transposons (CASTs) by analyzing metagenomic databases, revealing novel architectures and systems that co-opt different CRISPR-Cas effectors.

The paper profiles cleavage specificity and kinetics of CRISPR nucleases using NucleaSeq and CHAMP, finding engineered Cas9s show improved specificity while wtCas12a has similar specificity to wtCas9 in vitro.