Daan B Boltje

The paper presents three strategies for live feedback control during the fabrication of frozen-hydrated lamellae using cryogenic FIB-SEM, ensuring thickness and quality.

The paper demonstrates using optical interference in a cryogenic FIB-SEM-LM system to target lamella production beyond the diffraction limit for capturing virions in host cells.

The paper derives a depth-dependent axial scaling theory for light microscopy under refractive index mismatch and provides software to correct axial deformation in imaging data.

This paper introduces a new cryogenic microscope that combines fluorescence, ion beam, and electron beam imaging to improve the targeting and fabrication of lamellae for high-resolution imaging.