Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes
Wan Lin Yeo, Elena Heng, Lee Ling Tan, Yi Wee Lim, Yee Hwee Lim, Shawn S. Hoon, Huimin Zhao, Mingzi M. Zhang, Fong Tian Wong
The paper evaluates alternative CRISPR-Cas systems for genome editing in streptomycetes, showing that sth1Cas9, saCas9, and fnCpf1 enable efficient genomic modifications where SpCas9 fails.
Biotechnology and Bioengineering · May 15, 2019Peer-reviewedSynthetic Biology