Fong Tian Wong

The paper introduces MethylHDA, a method that combines helicase-dependent amplification with DNMT1 to preserve DNA methylation during targeted amplification.

This paper introduces a multimodal framework that links biosynthetic gene clusters and natural products by integrating genomic and chemical data through shared embeddings, enabling bidirectional retrieval and strain selection.

The paper integrates mass spectrometry and genomics data with language models to accelerate natural product discovery in microbial strains.

This paper compares two Megasphaera elsdenii strains, finding that JCM 35779 produces 5.8 times more hexanoic acid than JCM 1772 due to genomic differences affecting carbon flux.

The paper creates a fungal dataset of 518 strains from Singapore with taxonomic and visual phenotype data to aid in fungal classification and biodiversity studies.

This study demonstrates the use of spent mammalian culture media to produce recombinant FGF2-G3 in GRAS Lactococcus lactis, showing potential for sustainable protein production and cultivated meat growth.

This paper develops a hyper-porous hydrogel encapsulation method for microbial cells, demonstrating improved biocatalytic efficiency in co-culture fermentation.

This study optimizes CO2/H2 fermentation by Clostridium carboxidivorans sp. 624 using experimental and computational methods to enhance acid production.

This paper engineers Corynebacterium glutamicum to produce glycolic acid from ethylene glycol, achieving high titers through promoter engineering and a two-stage biotransformation strategy.

This paper characterizes and improves an 11-amino acid tag (NT11) to enhance recombinant protein expression in E. coli, doubling yields for FGF2 and hEGF.

This study uses protein language models to design sweeter and more thermostable brazzein variants, identifying mutations that improve heat resistance and sweetness.

This paper presents a cost-effective hybrid sequencing approach for assembling GC-rich Streptomyces genomes, enabling accurate biosynthetic gene cluster identification and revealing a new species.

The paper evaluates alternative CRISPR-Cas systems for genome editing in streptomycetes, showing that sth1Cas9, saCas9, and fnCpf1 enable efficient genomic modifications where SpCas9 fails.