A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM
Suzanne E. Enos, B. Cook, Hamidreza Rahmani, Sarah M. Narehood, Yizhou Li, Inga Christine Kuschnerus, Trevor H Redford, Peter Dukakis, Daniel Ji, Maxwell J. Bachochin, Danielle Ann Grotjahn, Mark A. Herzik
The paper introduces SurfACT, a surfactant cocktail that reduces air-water interface artifacts in cryoEM, improving particle distribution and map completeness for four proteins.
bioRxiv · Mar 18, 2026 · PreprintBiophysics