Mark A. Herzik

This study identifies glucose 6-phosphate (G6P) as a second trigger for Glk1p polymerization and shows that ADP causes filament disassembly, linking metabolic state to enzyme polymerization.

The paper introduces SurfACT, a surfactant cocktail that reduces air-water interface artifacts in cryoEM, improving particle distribution and map completeness for four proteins.

This study reveals the conformational dynamics of Tom70 through structural and computational analyses, showing how its allosteric network coordinates mitochondrial protein import and is modulated by viral interactions.

The paper identifies inhibitors of the GRPEL1-HSPA9 chaperone complex using tryptoline stereoprobes and shows they target non-orthosteric sites.

ANTIDOTE improves cryoEM particle sorting by using metadata-driven neural networks, enhancing reconstruction quality and reducing manual curation efforts.

This paper presents a streamlined method for (an)aerobic vitrification of oxygen-sensitive proteins using an automated device, enabling high-resolution cryoEM structure determination.

This paper characterizes the structure and dynamics of HSPB1 oligomers using biophysical and computational methods, revealing conformational heterogeneity and domain-specific interactions.

This study models the full-length APOBEC3B structure and finds that NTD-CTD interactions increase active site opening frequency.

The paper shows that short 5' UTRs in viral mRNAs are recognized by the IFIT2-IFIT3 complex, leading to translation inhibition and antiviral activity.

The paper determines the structure of human mortalin-GrpEL1 complexes using cryoEM and identifies mechanisms of nucleotide and substrate release in the Hsp70 system.

This paper describes the use of the chameleon device for tuning ice thickness to improve cryoEM grid quality and image quality.